A multitarget real-time PCR (MRT-PCR) for detection of Chlamydia trachomatis DNA was developed and validated. There were three targets for amplification in a single reaction: the cryptic plasmid (CP), the major outer membrane protein (MOMP) gene, and an internal control. The assay had the following characteristics: (i) detection and confirmation of the presence of C. Trachomatis DNA in a single reaction, (ii) detection of all genovars of C.

Download the free trial version below to get started. Double-click the downloaded file to install the software.

Trachomatis without any cross-reactivity with pathogenic bacteria or commensal organisms of the oropharynx and genital tract, (iii) a 95% probability of detection with three copies of MOMP and one copy of CP per reaction mixture, (iv) identification of the inhibition of amplification, (v) a quantitative dynamic range of 25 to 250,000 genome copies per reaction mixture, (vi) high intra- and interassay reproducibilities, and (vii) correct identification of all samples in the validation panel. There were 146 COBAS Amplicor PCR (Amplicor PCR)-positive samples and 122 Amplicor PCR-negative samples in the panel. MRT-PCR detected CP DNA alone in 6 (4%) Amplicor PCR-positive samples and both CP and MOMP DNAs in 140 (96%) of 146 Amplicor PCR-positive samples. The quantity of MOMP DNA in 95 (68%) of 140 samples was within the dynamic range of the assay.

The median C. Trachomatis load in these samples was 321 genome copies per reaction mixture (range, 26 to 40,137 genome copies per reaction mixture).

Due to the inclusion of two different C. Ea Cricket 07 Trainer Free Download. Trachomatis-specific targets, the assay confirmed 259 (97%) of 268 results in a single reaction. This assay could be used in the qualitative format for the routine detection of C. Trachomatis and in the quantitative format for study of the pathogenesis of C.

Trachomatis-associated diseases. The assay demonstrated the potential to eliminate the need for confirmatory testing in almost all samples, thus reducing the turnaround time and the workload. Chlamydia trachomatis is a bacterial infection of global public health significance (). It is an intracellular pathogen with 15 serotypes.

It causes trachoma (serotypes A, B, B 1, and C), lymphogranuloma venereum (serotypes L 1, L 2, and L 3), and oculogenital infections (serotypes D to K). There are many highly conserved nucleotide sequences in different chromosomal genes and in the cryptic plasmid (CP) of C. These sequences are used as targets for nucleic acid amplification tests (NAATs). NAATs have surpassed cell culture and antigen detection for the diagnosis of C. Trachomatis infections due to their enhanced sensitivities.